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  • Integrative Genomics
  • Integrative Genomics

    Research Activities

    With the aim of advancing personalized medicine and personalized prevention, we conduct genome analyses of biological specimens provided by cohort studies using next-generation sequencer. We also conduct omics analyses of protein and low-molecular metabolites using NMR, mass spectrometer, among other instruments. This data is processed using a super computer and other technologies.
    In parallel with these analyses, we are developing technologies for analyzing biological specimens, as well as software and information analysis methodologies for processing large quantities of data, including genome data.
    One of our goals is to create a benchmark genome reference panel of Japanese people through whole-genome analyses of more than 4,000 people.
    We have furthermore developed a tool for analyzing the genomes of Japanese people, which have allowed the analysis of quasi-whole-genome sequences of Japanese people (Japonica Array).

    Key Word: Massive parallel sequencing, Population genetics, Proteomics, Metabolomics, Bioinformatics, High performance computing

    Publications

    • Regional genetic differences among Japanese populations and performance of genotype imputation using whole-genome reference panel of the Tohoku Medical Megabank Project
    • The structural origin of metabolic quantitative diversity
    • iJGVD: an integrative Japanese genome variation database based on whole-genome sequencing

    Click HERE for more publications related to integrative genomics in ToMMo.

    Data Sharing

    Japanese Multi Omics Reference Panel (jMorp)

    “jMorp” is a database consisted of genome, metabolome and proteome data in plasma. Multiple omics analysis data obtained by ToMMo is integrated to “jMorp”, and opened to the researchers online.

    Genome

    We preformed around 3,500 whole genome sequencing and construct genome reference panel for Japanese population (3.5KJPNv2). Information on frequencies and locations of all found single-nucleotide variants (SNV) on the panel is available through the “jMorp”.

    You can search your interested SNVs on our platform by the reference SNP ID number (rsID), Gene Symbol and locate information on the international human genome reference sequence. All found SNVs frequencies in our reference panel are displayed and you can compare with those in other panels such as 1KGP.
    All the frequencies and locate information are downloadable by a single file for each chromosome.

    You can access the former versions of our genome reference panels, such as 1KJPN around 1,000 whole genome sequencing released as the first version of our panel in 2013, 2KJPN released in 2015, and 3.5KJPN released in 2017 on Integrative Japanese Genome Variation Database (iJGVD).


    after 3.5KJPNv2


    1KJPN, 2KJPN, 3.5KJPN 

     

    Achievement of Research product by Whole Genome Reference Panel: Japonica Array
    The “Japonica Array”, DNA microarray designed for genome analysis of Japanese population, is developed in 2014 by ToMMo, and it has been used for our genome analyses of tens of thousands of cohort participants. The array contains common SNPs in Japanese population as well as disease-related SNPs. Whole genome sequence can be imputed very precisely with our genome reference panel.

     

    Metabolome & Proteome

    On “jMorp”, the results of global metabolome analysis for metabolites in serum and proteome analysis are available. You can find the distribution and frequencies information for major metabolites for several thousand population and proteins for several hundred population.

    Metabolome data were measured by proton NMR and LC-MS, and proteome data were obtained by nanoLC-MS.

    Metabolome data obtained from more than 5,000 adult, and proteome data obtained from more than 500 adult.

    We measured several thousand metabolites including the uncharacterized ones and several hundred proteins by now. The data will be released after carefully checking each metabolite and protein in order. We also released correlation among metabolites and observed peptides of 256 abundant proteins.

    iMorp

    Japanese Reference Genome(JRG)

    ToMMo has constructed the Japanese reference genome v1 (JRGv1) with a long-read-type next generation sequencer, PacBio RSII (Pacific Biosciences), and the data is now available online.
    We repeatedly sequenced Japanese genomic DNA to obtain sequence information of 300-billion bases (as much as 100 times repeated sequencing of a genome). Then, we have applied a method of information science called de novo assembly, assembling the sequences from scratch with high accuracy. And finally we succeeded construction include 903 new insertion sequences (approximately a million bases in total) to the international human reference genome sequence GRCh38, and release of the present Japanese Reference Genome.
    Some sequences determined by long-read-type sequencer were not easily fit to the specific sites on GRCh38. We have collected such those sequences and released as decoyJRGv1. Now those were also be opened on the net.
    topix_JRG_LOGO
    Japanese Reference Genome

    Movies

    We use the next-generation genome sequencer to analyze the whole genome sequences of the part of cohort participants.

    Interviews

    -3/11, Disaster Medicine, & ToMMo: Perspectives from the Executive Director (Interview with Dr. Yamamoto, November 10th, 2015)

    Press Releases

    -Genetic Diversity of Enzymes Alters Metabolic Individuality (Sep. 2, 2016)
    -Rare variant discovery by deep whole-genome sequencing of 1,070 Japanese individuals (Aug. 21, 2015)
    -Japonica Array: Improved genotype imputation by designing a population-specific SNP array with 1,070 Japanese individuals (June 24, 2015)
    -Partial release of data from Tohoku Medical Megabank Project's Whole Genome Reference Panel (Sep. 5, 2014)
    -Tohoku Medical Megabank Organization high-accuracy whole genome sequencing of 1,000 healthy Japanese people: A base to search for the causes of diseases-Collection of over 15 million new gene polymorphisms- (Nov. 29, 2013)

    News

    -New version of Japanese Multi Omics Reference Panel "jMorp": 10,719 people Metabolome data are now available online (Sep. 6, 2018)
    -Expanded version of Japanese genome reference panel including mitochondria and X chromosome is available online (June 25, 2018)
    -New expanded version of Japanese Multi Omics Reference Panel "jMorp": 5,093 people Metabolome data are now available online (Oct. 3, 2017)
    -The locations and allele frequencies of SNVs of 3,554 Japanese individuals by whole genome sequences is available (Sep. 28, 2017)
    -Announcing release of JRG v2 and decoyJRGv2 (June 6,2017)
    -We enlarged Japanese Multi Omics Reference Panel "jMorp", 1,008 people Metabolome data are now online (Sep. 12, 2016)
    -Announcing release of JRG v1 (Aug. 25, 2016)
    -Release of locations and allele frequencies of all SNVs in whole genome sequences of 2,049 Japanese individuals (June 6, 2016)
    -Access for the locations and allele frequencies of all SNVs in the Whole Genome Reference Panel (Dec. 10, 2015)